One DBS card, each with six calibrator spots, packaged in resealable foil pouches with desiccant. The DBS card is Whatman #903 filter paper, on which whole blood spiked with different levels of SMN1, TREC and RNase P plasmid DNA is spotted. The TREC-SMN1-RNase P DBS Calibrator can be used with SCID-SMA Multiplex Real Time PCR assay